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Home • 3wk-both7 • Segmentation Information

Data Set Information

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  Specimen preparation
  Microscopy product
  Imaging product type
  Specimen description
  Imaging parameters

  2D image
  Reconstruction
  Segmentation

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Project Information

Microscopy product ID: 1009
Image basename: 3wk-both7

Project information
Project Experiment Subject group Subject Organism Tissue section or block View this project's published data sets View this project's tree structure

Project (P1230)
Project ID	P1230
Project name	Astrocyte Development
Project description	Postnatal development of protoplasmic astrocytes
Leader	Eric Bushong (Other projects)
Funding agency	NIH
Project start date	2002-02-01
Project end date
Collaborators	Maryann Martone, Mark Ellisman
Publications	Bushong EA, Martone ME, Ellisman MH. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86.

Experiment (23)
Experiment title	Morphology of astrocytes in 3 week old hippocampus
Experiment purpose	Examine the morphology of 3 week old astrocytes
Experiment date
Experimenter	Eric Bushong

Subject group (23)
Subject group name	NA
Group by	NA
Treatment	NA

Subject (23)
Species	rat
Scientific name	rattus norvegicus
Strain	Sprague Dawley

Organism (23)
Age class	juvenile
Age	3.0 weeks
Sex	male
Weight
Vendor
Cell Culture
Culture type
Date plated
Plating density
Number of passages
Media
Cultured by
Preparation
Cell type
Source
Tissue product storage
Cell line ID
Lot number
Notes

Tissue section or block (30)
Anatomical location	hippocampus
Tissue product storage	coverslipped
Microtome	vibratome
Thickness	100.0 um
Orientation	coronal

Specimen Preparation Information

Microscopy product ID: 1009
Image basename: 3wk-both7

Specimen Preparation
Tissue group type	NA
Ext file name	NA
Protocol used

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Microscopy Product Information

Microscopy product ID: 1009
Image basename: 3wk-both7

Microscopy product
Microscopy product id	1009
Image basename	3wk-both7
Instrument	Biorad Radiance2000
X Size	1024
Y Size	1024
Z Size
X Resolution	0.053623 um/pixel
Y Resolution	0.053623 um/pixel
Z Resolution
Create date
PURL	NA
Product type	optical section series
Microscope type	single photon confocal
Session name
Grid box count
Grid info.	N/A
Slide info.
Plane count	0
SRB ID	0

Imaging Product Types

Microscopy product ID: 1009
Image basename: 3wk-both7

Optical section series
Optical PSF file
Descriptions
Optical Z resolution	0.25 um
Cutting plane

Through focus series
Descriptions
Through PSF file
Z step

Specimen Description

Microscopy product ID: 1009
Image basename: 3wk-both7

Specimen description (1009, 3wk-both7)
System	central nervous system
Tissue
Organ	brain
Region	hippocampus
Subregions	CA1, stratum radiatum
Cell type	protoplasmic astrocyte
Cell id
Structure
Atlas coordinates
Atlas
Map location
Notes

Imaging Parameters

Microscopy product ID: 1009
Image basename: 3wk-both7

Light microscopy product (1009, 3wk-both7)
Coverslip thickness	1.0 um
Lens	Nikon
Lens magnification	60.0 X
Numerical aperture	1.4
Immersion medium	oil
Mounting medium	gelvatol
Refractive index	1.5

LM channels

Confocal

All channels

Confocal channel (10109)
Filter used
Fluorophor	Lucifer Yellow
Color	Green
Excitation wave length	428.0 nm
Emission wave length	536.0 nm
Laser type	Krypton-Argon
Notes
Specimen preparations View complete protocol

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2D Image Information

Microscopy product ID: 1009
Image basename: 3wk-both7

Downloadable data file description
Full-resolution version
Down-sampled version

Raw data info
Bit depth
Digitized by
Digitizing platform

Animation
No animation available.

Data files
Action	File type	File size	Description

WIB viewer
JPG view

Description:
No WIB image found.

Description:

id: 1009 - Click to go back to the summary page

Reconstruction Information

Microscopy product ID: 1009
Image basename: 3wk-both7

Reconstruction description
Reconstruction description
Full-resolution reconstruction file
Down-sampled version of the reconstruction file
Image map file
Reconstruction file format
Volume dimension
Voxel scale

More details

Animation
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Data files
Action	File type	File size	Description
Download	512x512 image	Unknown	Optical section series through adjacent protoplasmic astrocytes in rat hippocampal area CA1 intracellulaly injected Lucifer Yellow (green) and Alexa 568 (red) respectively, imaged with confocal microscopy
Download	Reconstruction data file	110.94MB	Not available

WIB viewer
JPG view

Description: Optical section series through adjacent protoplasmic astrocytes in rat hippocampal area CA1 intracellulaly injected Lucifer Yellow (green) and Alexa 568 (red) respectively, imaged with confocal microscopy

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Segmentation Information

Microscopy product ID: 1009
Image basename: 3wk-both7

Message

No segmentation data is available for this data set yet.