Description: Single tilt image at zero degress of tilt through a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form żonion skinż sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. Dark particles are 20 nm gold particles applied to the surface of the section to serve as fiducial marks for subsequent alignment.
Description: Single tilt image at zero degress of tilt through a lamellar structure observed in HeLa cells transfected with mutant connexin 50, imaged using intermediate voltage electron microscopy, and labeled using the tetracysteine-ReHAsH system and fluorescence photoooxidation. The concentric double membrane layers of the aggregate appear to form żonion skinż sheets that do not form a closed structure along the long axis. The differential staining between the outside and inside is most likely due to impeded diffusion of DAB into the interior of these accumulations. Dark particles are 20 nm gold particles applied to the surface of the section to serve as fiducial marks for subsequent alignment.
