| Leader |
Keun-Young Kim
(Other projects)
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| Collaborators |
David Gutmann, Eric Bushong, Mark Ellisman, Da-Yong Lee |
| Project Description |
Use of large scale imaging of the optic nerve and chiasm to characterize development of optic gliomas from neurofibromatosis-1 (Nf1) genetically-engineered mice |
Experiment Purpose |
The purpose of this study was to investigate the progression of changes in retinal ganglion cells and optic nerve glia in neurofibromatosis-1 (NF1) genetically-engineered mice with optic glioma. RGC were counted in whole retina preparations using high-resolution, mosaic confocal microscopy following their delineation by retrograde FluoroGold labeling. Then, we found reduced RGC numbers in Nf1+/¿GFAPCKO mice, supporting a model in which the combination of optic nerve Nf1 heterozygosity and glial cell Nf1 loss results in disrupted axonal-glial relationships, subsequently culminating in the degeneration of optic nerve axons and loss of their parent RGC neurons. |
| Instrument |
Olympus DSU Confocal Microscope |
| Channel information |
JSP Page
| Molecular target |
Chromagen |
Excitation λ |
Display color |
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Fluorogold in saline
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Fluorogold |
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| Publications |
Optic nerve dysfunction in a mouse model of neurofibromatosis-1 optic glioma.
Hegedus B, Hughes FW, Garbow JR, Gianino S, Banerjee D, Kim KY, Ellisman MH, Brantley MA Jr, Gutmann DH.
J Neuropathol Exp Neurol. 2009 May;68(5):542-51.
Ultrastructural characterization of the optic pathway in a mouse model of neurofibromatosis-1 optic glioma.
Kim KY, Ju WK, Hegedus B, Gutmann DH, Ellisman MH.
Neuroscience. 2010 Sep 29;170(1):178-88. Epub 2010 Jun 23. PMID: 20600672
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