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Home • nmodel • Specimen Preparation
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Specimen Preparation Information
Microscopy product ID: 8244
Image basename: nmodel
Specimen Preparation
Tissue group type Golgi impregnation
Ext file name
Protocol used Perfused with 4% PFA, 0.1% glut. Postfixed 1 hour in same fix on ice. Left hemisphere cut into 2 mm thick coronal slabs. Tissue placed into 2.3% potassium dichromate / 0.4% OsO4 for 3 days at room temp. Tissue blotted with filter paper and then placed in 0.7% silver nitrate in dark for 3 days. Tissue was dehydrated, infiltrated and embedded in Durcupan in Beem capsules.


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